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Nat Cell Biol. 2009 Aug;11(8):1039-42. doi: 10.1038/ncb1918. Epub 2009 Jul 13.

Differential requirements for actin during yeast and mammalian endocytosis.

Author information

1
Department of Molecular Biology and Biotechnology, University of Sheffield, Firth Court, Western Bank, Sheffield, S10 2TN, UK.

Abstract

Key features of clathrin-mediated endocytosis have been conserved across evolution. However, endocytosis in Saccharomyces cerevisiae is completely dependent on a functional actin cytoskeleton, whereas actin appears to be less critical in mammalian cell endocytosis. We reveal that the fundamental requirement for actin in the early stages of yeast endocytosis is to provide a strong framework to support the force generation needed to direct the invaginating plasma membrane into the cell against turgor pressure. By providing osmotic support, pressure differences across the plasma membrane were removed and this reduced the requirement for actin-bundling proteins in normal endocytosis. Conversely, increased turgor pressure in specific yeast mutants correlated with a decreased rate of endocytic patch invagination.

PMID:
19597484
PMCID:
PMC2875176
DOI:
10.1038/ncb1918
[Indexed for MEDLINE]
Free PMC Article

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