Structural profiling of disaccharides derived from RIP-extracted tissue HS using fluorescence detection. HS was purified from differing amounts of mouse kidney by RIP, and disaccharide compositions were profiled using HPLC and BODIPY fluorescence detection. Representative profiles are shown for data obtained from 100 (a), 10 (b), and 1 mg (c) of starting material (analysis was performed on one-half of each sample). Elution positions of the eight commonly occurring disaccharides are indicated. d, quantitative comparison of the compositional profiles expressed as the percentage of total disaccharides (mean ± S.D.; n = 3). Disaccharide standards are as follows: standard 1, UA-GlcNAc; standard 2, UA-GlcNAc(6S); standard 3, UA-GlcNS; standard 4, UA-GlcNS(6S); standard 5, UA(2S)-GlcNS; standard 6, UA(2S)-GlcNS(6S); standard 7, UA(2S)-GlcNAc; standard 8, UA(2S)-GlcNAc(6S).