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Exp Biol Med (Maywood). 2009 Oct;234(10):1212-9. doi: 10.3181/0812-RM-349. Epub 2009 Jul 13.

Hydrogen-rich saline protects myocardium against ischemia/reperfusion injury in rats.

Author information

1
Department of Diving Medicine, Faculty of Naval Medicine, Second Military Medical University, Shanghai, 200433, P. R. China. sunxjk@hotmail.com

Abstract

Protective effect of hydrogen (H(2)) gas on cardiac ischemia-reperfusion (I/R) injury has been demonstrated previously. This study was designed to test the hypothesis that hydrogen-rich saline (saline saturated with molecular hydrogen), which is easy to use, induces cardioprotection against ischemia (30 min) and reperfusion (24 h) injury in rats. Adult male Sprague-Dawley rats underwent 30-min occlusion of the left anterior descending (LAD) coronary artery and 24-h reperfusion. Intraperitoneal injection of hydrogen-rich saline before reperfusion significantly decreased plasma and myocardium malondialdehyde (MDA) concentration, decreased cardiac cell apoptosis, and myocardial 8-hydroxydeoxyguanosine (8-OHdG) in area at risk zones (AAR), suppressed the activity of caspase-3, and reduced infarct size. The heart function parameters including left ventricular systolic pressure (LVSP), left ventricular diastolic pressure (LVDP), +(dP/dt)(max) and -(dP/dt)(max) were also significantly improved 24 h after reperfusion. It is concluded that hydrogen-rich saline is a novel, simple, safe, and effective method to attenuate myocardial I/R injury.

PMID:
19596825
DOI:
10.3181/0812-RM-349
[Indexed for MEDLINE]

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