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Anal Biochem. 2009 Oct 15;393(2):176-81. doi: 10.1016/j.ab.2009.07.009. Epub 2009 Jul 28.

Fully automated protein purification.

Author information

1
Department of Chemistry, University of Toledo, Toledo, OH 43606, USA.

Abstract

Obtaining highly purified proteins is essential to begin investigating their functional and structural properties. The steps that are typically involved in purifying proteins can include an initial capture, intermediate purification, and a final polishing step. Completing these steps can take several days and require frequent attention to ensure success. Our goal was to design automated protocols that would allow the purification of proteins with minimal operator intervention. Separate methods have been produced and tested that automate the sample loading, column washing, sample elution and peak collection steps for ion exchange, metal affinity, hydrophobic interaction, and gel filtration chromatography. These individual methods are designed to be coupled and run sequentially in any order to achieve a flexible and fully automated protein purification protocol.

PMID:
19595984
PMCID:
PMC2757653
DOI:
10.1016/j.ab.2009.07.009
[Indexed for MEDLINE]
Free PMC Article

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