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Poult Sci. 2009 Aug;88(8):1773-8. doi: 10.3382/ps.2009-00054.

Production of an egg yolk antibody against Parietaria judaica 2 allergen.

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Università di Palermo, Sezione di Biologia e Genetica, Dipartimento di Biopatologia e Metodologie Biomediche, Palermo, Italy.


Specific antibodies are essential tools for studying proteins as well as for diagnostic research in biomedicine. The egg yolk of immunized chicken is an inexpensive source of high-quality polyclonal antibodies. The 12-kDa Parietaria judaica 2 allergen was expressed as a fusion protein and was used to immunize Leghorn chickens. In this paper, we show, using 2-dimensional gel electrophoresis and immunoblotting, that chicken antibodies raised against a recombinant allergen can be used to recognize similar proteins from a pollen raw extract. Allergen identity was confirmed by nanoLC-nanospray-tandem mass spectrometry analysis. Our data demonstrate for the first time that a synergistic combination of molecular biology, 2-dimensional PAGE, and use of nonmammalian antibodies represents a powerful tool for reliable identification of allergens.

[Indexed for MEDLINE]

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