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Poult Sci. 2009 Aug;88(8):1773-8. doi: 10.3382/ps.2009-00054.

Production of an egg yolk antibody against Parietaria judaica 2 allergen.

Author information

1
Università di Palermo, Sezione di Biologia e Genetica, Dipartimento di Biopatologia e Metodologie Biomediche, Palermo, Italy. ricale@unipa.it

Abstract

Specific antibodies are essential tools for studying proteins as well as for diagnostic research in biomedicine. The egg yolk of immunized chicken is an inexpensive source of high-quality polyclonal antibodies. The 12-kDa Parietaria judaica 2 allergen was expressed as a fusion protein and was used to immunize Leghorn chickens. In this paper, we show, using 2-dimensional gel electrophoresis and immunoblotting, that chicken antibodies raised against a recombinant allergen can be used to recognize similar proteins from a pollen raw extract. Allergen identity was confirmed by nanoLC-nanospray-tandem mass spectrometry analysis. Our data demonstrate for the first time that a synergistic combination of molecular biology, 2-dimensional PAGE, and use of nonmammalian antibodies represents a powerful tool for reliable identification of allergens.

PMID:
19590094
DOI:
10.3382/ps.2009-00054
[Indexed for MEDLINE]

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