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J Biol Chem. 2009 Aug 28;284(35):23182-6. doi: 10.1074/jbc.C109.038075. Epub 2009 Jul 8.

FANCI binds branched DNA and is monoubiquitinated by UBE2T-FANCL.

Author information

1
Department of Molecular Biophysics, Yale University School of Medicine, New Haven, Connecticut 06520, USA.

Abstract

FANCI is integral to the Fanconi anemia (FA) pathway of DNA damage repair. Upon the occurrence of DNA damage, FANCI becomes monoubiquitinated on Lys-523 and relocalizes to chromatin, where it functions with monoubiquitinated FANCD2 to facilitate DNA repair. We show that FANCI and its C-terminal fragment possess a DNA binding activity that prefers branched structures. We also demonstrate that FANCI can be ubiquitinated on Lys-523 by the UBE2T-FANCL pair in vitro. These findings should facilitate future efforts directed at elucidating molecular aspects of the FA pathway.

PMID:
19589784
PMCID:
PMC2749091
DOI:
10.1074/jbc.C109.038075
[Indexed for MEDLINE]
Free PMC Article

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