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Indian J Med Sci. 2009 May;63(5):187-94. doi: 10.4103/0019-5359.53164.

Vitamin D receptor (FokI, BsmI and TaqI) gene polymorphisms and type 2 diabetes mellitus: a North Indian study.

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1
Endocrinology Division, CDRI, Lucknow, India.

Abstract

BACKGROUND:

The vitamin D receptor (VDR) gene is a candidate gene for susceptibility to several diseases. Studies on association between VDR polymorphisms and risk of type 2 diabetes (T2DM) in different ethnic populations are yet inconclusive.

AIMS:

This study was conducted to evaluate association between VDR polymorphisms and genetic susceptibility to T2DM in the north Indian population.

SETTINGS AND DESIGN:

One hundred clinically diagnosed T2DM patients and 160 healthy controls from the north Indian population were recruited for genetic association study.

MATERIALS AND METHODS:

Genomic DNA was extracted from blood and genotyped for the single nucleotide polymorphism SNPs of FokI (T/C) [rs2228570], BsmI (A/G) [rs1544410] and TaqI (C/T) [rs731236] by polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) analysis.

STATISTICAL ANALYSIS USED:

Genotype distribution and allelic frequencies were compared between patients and controls. Mean values and odds ratios (ORs) with 95% confidence interval (CI) were calculated using SPSS software (version 15.0).

RESULTS:

The genotype distribution, allele and haplotype frequencies of VDR polymorphism did not differ significantly between patients and controls. Mean age and waist-hip ratio of patients were found to be associated with VDR polymorphism. Combination studies showed FFBbtt increased the risk of T2DM in north Indians.

CONCLUSIONS:

Our data suggest that VDR gene polymorphism in combination of genotypes is associated with the risk of T2DM and thus requires further studies as a probable genetic risk marker for T2DM.

PMID:
19584489
DOI:
10.4103/0019-5359.53164
[Indexed for MEDLINE]
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