Format

Send to

Choose Destination
See comment in PubMed Commons below
Virol J. 2009 Jul 7;6:95. doi: 10.1186/1743-422X-6-95.

Improvement of a real-time RT-PCR assay for the detection of enterovirus RNA.

Author information

  • 1Department of Microbiology, ZNA Hospitals, site Middelheim, Lindendreef 1, 2020 Antwerp, Belgium. marianpiqueur@hotmail.com

Abstract

We describe an improvement of an earlier reported real-time RT-PCR assay for the detection of enterovirus RNA, based on the 5' exonuclease digestion of a dual-labeled fluorogenic probe by Taq DNA polymerase. A different extraction method, real-time RT-PCR instrument and primer set were evaluated. Our data show that the optimized assay yields a higher sensitivity and reproducibility and resulted in a significant reduced hands-on time per sample.

PMID:
19583870
PMCID:
PMC2716329
DOI:
10.1186/1743-422X-6-95
[PubMed - indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for BioMed Central Icon for PubMed Central
    Loading ...
    Support Center