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Electrophoresis. 2009 Jun;30(12):2100-9. doi: 10.1002/elps.200900144.

CE of dsDNA in low-molecular-weight polyethylene oxide solutions.

Author information

1
Department of Chemistry, Imperial College London, Exhibition Road, South Kensington, London, UK.

Abstract

To realise effective size separations of nucleic acid fragments using CE, gel-based matrices are commonly employed. The separation of label-free dsDNA ladders and plasmid fragments in an uncross-linked semi-dilute poly (ethylene) oxide solution using multi-pixel UV detection at 254 nm is reported. Improvements in the sensitivity of UV detection of dsDNA using signal averaging over multiple pixels is demonstrated. Separations performed using a diode array detector also allow the progress of the separation to be monitored as a function of time. Several polymers were examined including methyl cellulose, linear polyacrylamide, hydroxy (propyl) methylcellulose and polyethylene oxide. Operations parameters investigated included UV transparency, self-coating capacity and separation efficiency. The results show complete resolution of all fragments under a range of conditions, including short separation lengths.

PMID:
19582710
DOI:
10.1002/elps.200900144
[Indexed for MEDLINE]

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