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Opt Express. 2008 Nov 10;16(23):19260-70.

Superresolution digital holographic microscopy for three-dimensional samples.

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Departamento de Optica, Universitat de Valencia, Burjassot, Spain.


An approach that allows superresolution imaging of three-dimensional (3-D) samples by numerical refocusing is presented in the field of digital holographic microscopy. Based on the object's spectrum shift produced by tilted illumination, we present a time multiplexing superresolved approach to overcome the Abbe's diffraction limit. The proposed approach uses a microscope in a Mach-Zehnder interferometric architecture with the particularity that the output plane does not coincide with the image plane. Thus, a set of off-axis non-image plane holograms are sequentially recorded for every tilted beam used in the illumination stage. After that and by using simple digital post-processing and numerical reconstruction, a 3-D superresolved sample volume is reconstructed slice-by- slice in terms of the definition of a synthetic aperture (SA) that expands the cutoff frequency of the microscope lens. Experimental results showing the capabilities of the proposed approach are presented.

[Indexed for MEDLINE]

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