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Ann Clin Biochem. 1991 Sep;28 ( Pt 5):504-8.

Measurement of malondialdehyde in plasma by high performance liquid chromatography with fluorimetric detection.

Author information

1
Department of Clinical Biochemistry, Queen's University of Belfast, UK.

Abstract

The thiobarbituric acid test for malondialdehyde is widely used as an indicator of lipid peroxidation and free radical activity. We describe a simple and sensitive high performance liquid chromatographic (HPLC) method for measuring the malondialdehyde-thiobarbituric acid adduct using fluorimetric detection. Extraction into organic solvent or other complex sample preparation is not required and the assay is sensitive to less than 0.05 mumol/L. This method could easily be applied to any setting where a measure of lipid peroxidation is required.

PMID:
1958055
DOI:
10.1177/000456329102800514
[Indexed for MEDLINE]

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