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Inflamm Res. 2009 Dec;58(12):899-908. doi: 10.1007/s00011-009-0063-1. Epub 2009 Jul 5.

Curcumin inhibits pro-inflammatory mediators and metalloproteinase-3 production by chondrocytes.

Author information

1
Institute of Pathology, CHU Sart-Tilman, Li├Ęge, Belgium. mmathy@ulg.ac.be

Abstract

OBJECTIVE AND DESIGN:

This study aims to investigate the effects of curcumin (Cur) on the extracellular matrix protein metabolism of articular chondrocytes and on their production of inflammatory mediators.

METHODS:

Human chondrocytes in alginate beads and human cartilage explants were cultured in the absence or in the presence of interleukin (IL)-1beta (10(-11) M) and with or without Cur (5-20 microM). Nitric oxide (NO) synthesis was measured by the Griess spectrophotometric method; prostaglandin (PG) E(2) by a specific radioimmunoassay; and IL-6, IL-8, aggrecan (Agg), matrix metalloproteinase (MMP)-3, and tissue inhibitor of metalloproteinase (TIMP)-1 by specific enzyme-amplified immunoassays. Proteoglycan degradation was evaluated by the release of (35)S-glycosaminoglycans (GAG) from human cartilage explants.

RESULTS:

In alginate beads and cartilage explant models, Cur inhibited the basal and the IL-1beta-stimulated NO, PGE(2), IL-6, IL-8, and MMP-3 production by human chondrocytes in a concentration-dependent manner. The TIMP-1 and the Agg productions were not modified. In the basal condition, (35)S-GAG release from cartilage explants was decreased by Cur.

CONCLUSIONS:

Curcumin was a potent inhibitor of the production of inflammatory and catabolic mediators by chondrocytes, suggesting that this natural compound could be efficient in the treatment of osteoarthritis.

PMID:
19579007
DOI:
10.1007/s00011-009-0063-1
[Indexed for MEDLINE]

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