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Nat Methods. 2009 Aug;6(8):577-9. doi: 10.1038/nmeth.1346. Epub 2009 Jul 5.

Conditional and reversible disruption of essential herpesvirus proteins.

Author information

1
Department of Virology, Hannover Medical School, Hannover, Germany.

Abstract

Elucidating the function of essential proteins of complex pathogenic viruses is impeded by a paucity of complementing systems. By fusing a destabilizing domain of the FK506-binding protein to essential cytomegalovirus proteins, we generated virus mutants in which amounts of fusion proteins and viral growth can be regulated by the synthetic ligand shield-1. This conditional approach will greatly facilitate the analysis of gene functions of herpesviruses and viruses of other families.

PMID:
19578384
DOI:
10.1038/nmeth.1346
[Indexed for MEDLINE]

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