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Nat Methods. 2009 Aug;6(8):585-7. doi: 10.1038/nmeth.1347. Epub 2009 Jul 5.

Mass spectrometry of membrane transporters reveals subunit stoichiometry and interactions.

Author information

1
Department of Chemistry, University of Cambridge, Cambridge, UK.

Abstract

We describe a general mass spectrometry approach to determine subunit stoichiometry and lipid binding in intact membrane protein complexes. By exploring conditions for preserving interactions during transmission into the gas phase and for optimally stripping away detergent, by subjecting the complex to multiple collisions, we released the intact complex largely devoid of detergent. This enabled us to characterize both subunit stoichiometry and lipid binding in 4 membrane protein complexes.

PMID:
19578383
PMCID:
PMC4066579
DOI:
10.1038/nmeth.1347
[Indexed for MEDLINE]
Free PMC Article

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