Format

Send to

Choose Destination
See comment in PubMed Commons below
Biochem Biophys Res Commun. 2009 Sep 18;387(2):289-93. doi: 10.1016/j.bbrc.2009.07.002. Epub 2009 Jul 3.

Mechanosensitive promoter region in the human HB-GAM gene.

Author information

1
Institute of Orthopedic Research and Biomechanics, University of Ulm, Germany. astrid.liedert@uni-ulm.de

Abstract

Mechanical loading is essential for maintaining bone mass in the adult skeleton. However, the underlying process of the transfer of the physical stimulus into a biochemical response, which is termed mechanotransduction is poorly understood. Mechanotransduction results in the modulation of gene expression through specific transcription factor binding sites in the promoter region of mechanosensitive genes. In the present study, we demonstrate that the expression of HB-GAM, which is known to have stimulating effects on osteogenic differentiation, is rapidly induced by mechanical loading in hMSC-TERT4 cells. Analysis of the human HB-GAM gene upstream regulatory region with luciferase reporter gene assays revealed that the upregulation of HB-GAM expression occurred at the transcriptional level and was mainly dependent on the HB-GAM promoter region most upstream containing three potential AP-1 binding motifs.

PMID:
19577542
DOI:
10.1016/j.bbrc.2009.07.002
[Indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Support Center