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J Biosci Bioeng. 2009 Jul;108(1):1-4. doi: 10.1016/j.jbiosc.2009.02.007.

Methods for energizing cell-free protein synthesis.

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1
Department of Fine Chemical Engineering and Chemistry, Chungnam National University, Daejeon 305-764, Republic of Korea.

Abstract

The productivity of cell-free protein synthesis depends critically upon the efficiency of ATP regeneration. In addition, the expensive energy sources used in the conventional cell-free protein synthesis systems account for the major part of the overall cost for the expression of proteins. Therefore, it is essential to implement a cheaper and more efficient ATP regeneration method in order to make cell-free protein synthesis a viable option for industrial protein production. In this article, we review the recent progress in the methodologies for supplying ATP during cell-free protein synthesis in E. coli extracts.

PMID:
19577183
DOI:
10.1016/j.jbiosc.2009.02.007
[Indexed for MEDLINE]
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