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Otolaryngol Head Neck Surg. 2009 Jul;141(1):52-8. doi: 10.1016/j.otohns.2009.01.041. Epub 2009 Mar 12.

Molecular markers of micrometastasis in oral cavity carcinomas.

Author information

1
Department of Otolaryngology, University of Miami, Miami, FL 33136, USA. rgermani@med.miami.edu

Abstract

OBJECTIVE:

To examine the expression of candidate markers for micrometastasis.

STUDY DESIGN:

Cross-sectional analysis of subjects with oral cavity carcinomas who underwent sentinel lymph node biopsy (SLNB) and subsequent immunohistochemical (IHC) analysis.

SUBJECTS AND METHODS:

Two groups were identified based on SLNB status: negative SLNB (19/30) and positive SLNB (11/30). Specimens underwent IHC using conjugated monoclonal antibodies for membrane type-1 matrix metalloproteinase (MT1-MMP), CD44, focal adhesion kinase-1, and E-cadherin. Staining results were evaluated to determine if a particular marker was associated with SLNB status or other histopathologic prognosticators.

RESULTS:

For MT1-MMP, 21 percent (3/14) of evaluable specimens stained positively in the SLNB(-) group and 67 percent (4/6) stained positively in the SLNB(+) group (P=0.12). No statistically significant association was seen between any marker's staining pattern and SLNB status alone. When MT1-MMP staining was evaluated in tumors with SLNB(+) or perineural invasion (PNI) present on histopathology, six of nine specimens (67%) stained positively for MT1-MMP, vs one of 11 (9%) in specimens lacking either negative prognosticator (P=0.016, RR=7.33).

CONCLUSION:

Preliminary results suggest that MT1-MMP positivity in primary tumor specimens may identify aggressive tumor types, evidenced by the presence of micrometastasis or PNI.

PMID:
19559958
DOI:
10.1016/j.otohns.2009.01.041
[Indexed for MEDLINE]
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