Format

Send to

Choose Destination
Vaccine. 2009 Oct 30;27(46):6454-9. doi: 10.1016/j.vaccine.2009.06.017. Epub 2009 Jun 24.

Improved antibiotic-free DNA vaccine vectors utilizing a novel RNA based plasmid selection system.

Author information

1
Nature Technology Corporation, 4701 Innovation Drive, Lincoln, NE 68521, USA.

Abstract

To ensure safety, regulatory agencies recommend elimination of antibiotic resistance markers from therapeutic and vaccine plasmid DNA vectors. Here, we describe the development and application of a novel antibiotic-free selection system. Vectors incorporate and express a 150 bp RNA-OUT antisense RNA. RNA-OUT represses expression of a chromosomally integrated constitutively expressed counter-selectable marker (sacB), allowing plasmid selection on sucrose. Sucrose selectable DNA vaccine vectors combine antibiotic-free selection with highly productive fermentation manufacturing (>1g/L plasmid DNA yields), while improving in vivo expression of encoded proteins and increasing immune responses to target antigens. These vectors are safer, more potent, alternatives for DNA therapy or vaccination.

PMID:
19559109
PMCID:
PMC2767433
DOI:
10.1016/j.vaccine.2009.06.017
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Elsevier Science Icon for PubMed Central
Loading ...
Support Center