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J Gen Virol. 2009 Oct;90(Pt 10):2375-80. doi: 10.1099/vir.0.013250-0. Epub 2009 Jun 24.

High-throughput sequence analysis of variants of human cytomegalovirus strains Towne and AD169.

Author information

1
MRC Virology Unit, Institute of Virology, Church Street, Glasgow G11 5JR, UK.

Abstract

The genomes of commonly used variants of human cytomegalovirus (HCMV) strains Towne and AD169 each contain a substantial mutation in which a region (U(L)/b') at the right end of the long unique region has been replaced by an inverted duplication of a region from the left end of the genome. Using high-throughput technology, we have sequenced HCMV strain Towne (ATCC VR-977) and confirmed the presence of two variants, one exhibiting the replacement in U(L)/b' and the other intact in this region. Both variants are mutated in genes RL13, UL1, UL40, UL130, US1 and US9. We have also sequenced a novel AD169 variant (varUC) that is intact in U(L)/b' except for a small deletion that affects genes UL144, UL142, UL141 and UL140. Like other AD169 variants, varUC is mutated in genes RL5A, RL13, UL36 and UL131A. A subpopulation of varUC contains an additional deletion affecting genes IRS1, US1 and US2.

PMID:
19553388
PMCID:
PMC2885757
DOI:
10.1099/vir.0.013250-0
[Indexed for MEDLINE]
Free PMC Article

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