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J Virol. 2009 Sep;83(17):9018-21. doi: 10.1128/JVI.00971-09. Epub 2009 Jun 24.

Is single-strand conformation polymorphism analysis of the full 5' untranslated region an adequate approach to study hepatitis C virus quasispecies distribution?

Author information

1
Laboratorio de Virología Molecular, Instituto Milenio de Inmunología e Inmunoterapia, and Jeune Equipe Associée à l'IRD, Centro de Investigaciones Médicas, Facultad de Medicina, Pontificia Universidad Católica de Chile, Marcoleta 391, Santiago, Chile.

Abstract

Single-strand conformation polymorphism (SSCP) analysis is used by many laboratories to study the quasispecies distribution of the hepatitis C virus (HCV). Here we question the validity of this experimental approach, as conclusions are drawn from the analysis of the migration patterns of two ssDNA molecules and not from RNA. Using previously characterized mutants of the HCV 5' untranslated regions, we show that contrary to what has been predicted, SSCP migration patterns of DNA amplicons with differences in their nucleotide sequences generated from the full 5' UTR of HCV are not necessarily unique.

PMID:
19553315
PMCID:
PMC2738205
DOI:
10.1128/JVI.00971-09
[Indexed for MEDLINE]
Free PMC Article

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