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Exp Oncol. 2009 Jun;31(2):92-6.

Expression profile of nuclear receptors upon Epstein -- Barr virus induced B cell transformation.

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  • 1Department of Microbiology, Tumor and Cell Biology (MTC), Karolinska Institute, Stockholm S17177, Sweden.

Abstract

BACKGROUND:

Infection of human B cells with Epstein - Barr virus (EBV) induces metabolic activation, morphological transformation, cell proliferation and eventual immortalization.

AIM:

To identify the nuclear receptors, which are the cellular interaction partners of EBNAs, that will help to elucidate the mechanism of B cell transformation.

METHODS:

We have compared the nuclear receptor profile in the naïve and EBV-transformed B-lymphocytes, using TaqMan LDA microfluidic card technology.

RESULTS:

Out of 48 nuclear receptor, 17 showed differential expression at the mRNA level. The expression of 5 genes was elevated in EBV-transformed cells, whereas 12 genes were downregulated in lymphoblastoid cells (LCLs). 7 genes were studied at the protein level; 2 genes were up regulated (Nr2F2 and RARA) and 4 genes were down regulated (ERB, NUR77, PPARG, and VDR) in LCLs.

CONCLUSION:

The nuclear receptor profiling on EBV infected B cells showed alterations of nuclear receptors expression at both mRNA and protein levels compared with non infected peripheral blood cells. Further analysis on a possible role of each nuclear receptor in EBV induced cell transformation should be performed.

PMID:
19550398
[PubMed - indexed for MEDLINE]
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