Format

Send to

Choose Destination
J Biosci. 2009 Jun;34(2):293-303.

Rapid aggregation and assembly in aqueous solution of A beta (25-35) peptide.

Author information

1
Department of Molecular Biology, University of Siena, Siena, Italy.

Abstract

The highly toxic A beta (25-35) is a peculiar peptide that differs from all the other commonly studied beta-amyloid peptides because of its extremely rapid aggregation properties and enhanced neurotoxicity. We investigated A beta (25-35) aggregation in H2O at pH 3.0 and at pH 7.4 by means of in-solution analyses. Adopting UV spectroscopy, Congo red spectrophotometry and thioflavin T fluorimetry, we were able to quantify, in water, the very fast assembling time necessary for A beta (25-35) to form stable insoluble aggregates and their ability to seed or not seed fibril growth. Our quantitative results, which confirm a very rapid assembly leading to stable insoluble aggregates of A beta (25-35) only when incubated at pH 7.4, might be helpful for designing novel aggregation inhibitors and to shed light on the in vivo environment in which fibril formation takes place.

PMID:
19550045
[Indexed for MEDLINE]
Free full text

Supplemental Content

Full text links

Icon for Indian Academy of Sciences
Loading ...
Support Center