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Clin Cancer Res. 2009 Jul 1;15(13):4292-8. doi: 10.1158/1078-0432.CCR-09-0599. Epub 2009 Jun 23.

Urine analysis and protein networking identify met as a marker of metastatic prostate cancer.

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1
Radiation Oncology Branch, National Cancer Institute, H. Lee Moffitt Cancer Center, Tampa, Florida, USA.

Abstract

PURPOSE:

Metastatic prostate cancer is a major cause of death of men in the United States. Expression of met, a receptor tyrosine kinase, has been associated with progression of prostate cancer.

EXPERIMENTAL DESIGN:

To investigate met as a biomarker of disease progression, urinary met was evaluated via ELISA in men with localized (n = 75) and metastatic (n = 81) prostate cancer. Boxplot analysis was used to compare the distribution of met values between each group. We estimated a receiver operating characteristic curve and the associated area under the curve to summarize the diagnostic accuracy of met for distinguishing between localized and metastatic disease. Protein-protein interaction networking via yeast two-hybrid technology supplemented by Ingenuity Pathway Analysis and Human Interactome was used to elucidate proteins and pathways related to met that may contribute to progression of disease.

RESULTS:

Met distribution was significantly different between the metastatic group and the group with localized prostate cancer and people with no evidence of cancer (P < 0.0001). The area under the curve for localized and metastatic disease was 0.90, with a 95% confidence interval of 0.84 to 0.95. Yeast two-hybrid technology, Ingenuity Pathway Analysis, and Human Interactome identified 89 proteins that interact with met, of which 40 have previously been associated with metastatic prostate cancer.

CONCLUSION:

Urinary met may provide a noninvasive biomarker indicative of metastatic prostate cancer and may be a central regulator of multiple pathways involved in prostate cancer progression.

PMID:
19549766
DOI:
10.1158/1078-0432.CCR-09-0599
[Indexed for MEDLINE]
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