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Mol Imaging Biol. 2010 Jan-Feb;12(1):42-53. doi: 10.1007/s11307-009-0240-1. Epub 2009 Jun 23.

Bioluminescence imaging in mouse models quantifies beta cell mass in the pancreas and after islet transplantation.

Author information

1
Vanderbilt University, Institute of Imaging Science, Nashville, TN, USA.

Abstract

PURPOSE:

We developed a mouse model that enables non-invasive assessment of changes in beta cell mass.

PROCEDURES:

We generated a transgenic mouse expressing luciferase under control of the mouse insulin I promoter [mouse insulin promoter-luciferase-Vanderbilt University (MIP-Luc-VU)] and characterized this model in mice with increased or decreased beta cell mass and after islet transplantation.

RESULTS:

Streptozotocin-induced, diabetic MIP-Luc-VU mice had a progressive decline in bioluminescence that correlated with a decrease in beta cell mass. MIP-Luc-VU animals fed a high-fat diet displayed a progressive increase in bioluminescence that reflected an increase in beta cell mass. MIP-Luc-VU islets transplanted beneath the renal capsule or into the liver emitted bioluminescence proportional to the number of islets transplanted and could be imaged for more than a year.

CONCLUSIONS:

Bioluminescence in the MIP-Luc-VU mouse model is proportional to beta cell mass in the setting of increased and decreased beta cell mass and after transplantation.

PMID:
19548035
PMCID:
PMC3822006
DOI:
10.1007/s11307-009-0240-1
[Indexed for MEDLINE]
Free PMC Article

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