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Nat Methods. 2009 Jul;6(7):497-9. doi: 10.1038/nmeth.1344. Epub 2009 Jun 21.

Simultaneous detection of many T-cell specificities using combinatorial tetramer staining.

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1
Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, California, USA.

Abstract

The direct detection of antigen-specific T cells using tetramers of soluble peptide-major histocompatibilty complex (pMHC) molecules is widely used in both basic and clinical immunology. However, the number of specificities that can be assessed simultaneously has been a major limitation. Here we describe and validate a method using combinations of fluorescent pMHC tetramers to simultaneously detect and enrich for many (>or=15) T-cell specificities in a single human blood sample.

PMID:
19543286
PMCID:
PMC2731062
DOI:
10.1038/nmeth.1344
[Indexed for MEDLINE]
Free PMC Article
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