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Curr Opin Neurol. 2009 Aug;22(4):379-86. doi: 10.1097/WCO.0b013e32832d9b86.

Genetic dissection of the mouse CNS using magnetic resonance microscopy.

Author information

1
Center for In Vivo Microscopy, Department of Radiology, Duke University Medical Center, Durham, North Carolina, USA.

Abstract

PURPOSE OF REVIEW:

Advances in magnetic resonance microscopy (MRM) make it practical to map gene variants responsible for structural variation in brains of many species, including mice and humans. We review results of a systematic genetic analysis of MRM data using as a case study a family of well characterized lines of mice.

RECENT ADVANCES:

MRM has matured to the point that we can generate high contrast, high-resolution images even for species as small as a mouse, with a brain merely 1/3000th the size of humans. We generated 21.5-micron data sets for a diverse panel of BXD mouse strains to gauge the extent of genetic variation, and as a prelude to comprehensive genetic and genomic analyses. Here we review MRM capabilities and image segmentation methods; heritability of brain variation; covariation of the sizes of brain regions; and correlations between MRM and classical histological data sets.

SUMMARY:

The combination of high throughput MRM and genomics will improve our understanding of the genetic basis of structure-function correlations. Sophisticated mouse models will be critical in converting correlations into mechanisms and in determining genetic and epigenetic causes of differences in disease susceptibility.

PMID:
19542887
PMCID:
PMC2734144
DOI:
10.1097/WCO.0b013e32832d9b86
[Indexed for MEDLINE]
Free PMC Article

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