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J Ethnopharmacol. 2009 Aug 17;125(1):90-6. doi: 10.1016/j.jep.2009.06.009. Epub 2009 Jun 18.

Drosera rotundifolia and Drosera tokaiensis suppress the activation of HMC-1 human mast cells.

Author information

1
School of Agriculture, Tokai University, Kawayou, Minamiaso-mura, Kumamoto 869-1404, Japan.

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE:

Several Northern Hemisphere Drosera species have been used in the therapy of respiratory tract infections as the traditional medicine Droserae Herba.

AIM OF THE STUDY:

To determine the anti-inflammatory effects of Drosera species and to investigate a substitute material for Droserae Herba, we examined the effect of extracts of Drosera rotundifolia, Drosera tokaiensis and Drosera spatulata on activated T cell membrane (aTc-m)-induced inflammatory gene expression in HMC-1 human mast cells.

MATERIALS AND METHODS:

Drosera rotundifolia, Drosera spatulata and Drosera tokaiensis were collected in Japan. Herbs were extracted with 80% EtOH, and subsequently applied to OASIS HLB column. HMC-1 cells were treated with each Drosera column-adsorbed fraction for 15min, and subsequently added to aTc-m and incubated for 16h. Inflammatory gene and protein expressions were determined by DNA microarray, RT-PCR and Western blotting.

RESULTS:

Drosera rotundifolia and Drosera tokaiensis fractions, but not the Drosera spatulata fraction, suppressed inflammatory gene expression induced by aTc-m in HMC-1 cells.

CONCLUSIONS:

Drosera rotundifolia and Drosera tokaiensis suppressed activation of HMC-1 cells induced by aTc-m. Since the Drosera tokaiensis fraction was more effective than the traditionally used Drosera rotundifolia, Drosera tokaiensis is a likely substitute as a source of Droserae Herba.

PMID:
19540325
DOI:
10.1016/j.jep.2009.06.009
[Indexed for MEDLINE]

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