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FEBS Lett. 2009 Jul 21;583(14):2333-8. doi: 10.1016/j.febslet.2009.06.024. Epub 2009 Jun 18.

Evidence for a degradosome-like complex in the mitochondria of Trypanosoma brucei.

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1
Depatment of Microbiology and Immunology, Witebsky Center for Microbial Pathogenesis and Immunology, SUNY Buffalo School of Medicine, Buffalo, NY 14214, USA.

Abstract

Mitochondrial RNA turnover in yeast involves the degradosome, composed of DSS-1 exoribonuclease and SUV3 RNA helicase. Here, we describe a degradosome-like complex, containing SUV3 and DSS-1 homologues, in the early branching protozoan, Trypanosoma brucei. TbSUV3 is mitochondrially localized and co-sediments with TbDSS-1 on glycerol gradients. Co-immunoprecipitation demonstrates that TbSUV3 and TbDSS-1 associate in a stable complex, which differs from the yeast degradosome in that it is not stably associated with mitochondrial ribosomes. This is the first report of a mitochondrial degradosome-like complex outside of yeast. Our data indicate an early evolutionary origin for the mitochondrial SUV3/DSS-1 containing complex.

PMID:
19540236
PMCID:
PMC2723791
DOI:
10.1016/j.febslet.2009.06.024
[Indexed for MEDLINE]
Free PMC Article
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