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J Dermatol Sci. 2009 Sep;55(3):185-92. doi: 10.1016/j.jdermsci.2009.05.005. Epub 2009 Jun 18.

Identification of the major allergen of Malassezia globosa relevant for atopic dermatitis.

Author information

1
Department of Immunobiology, Meiji Pharmaceutical University, Tokyo, Japan.

Abstract

BACKGROUND:

Malasseziaglobosa constitutes a part of the normal flora of human skin, but may induce IgE production in atopic dermatitis (AD). However, information on M.globosa allergens is scant.

OBJECTIVE:

To identify the major M. globosa allergens by using proteomic analysis.

METHODS:

Immunoglobulin E (IgE) immunoblotting and cross-inhibition tests for M. globosa allergens were performed using sera from AD patients and control subjects. These allergens were identified and characterized using the proteomics approach involving a combination of two-dimensional (2D) electrophoresis, mass spectrometry, and bioinformatics tools. We cloned the cDNA of this allergen using sequences obtained by 5'- and 3'-rapid amplification of cDNA ends polymerase chain reaction.

RESULTS:

The sera of the AD patients had IgE-reactive 40-45-kDa protein components. By 2D immunoblotting, we detected a 42-kDa protein spot with an isoelectric point (pI) of 4.8; the protein was highly reactive to IgE and was designated MGp42. Full-length MGp42 cDNA contained a 1908-bp open reading frame encoding 635 amino acid residues (calculated molecular mass, 69.7kDa; pI, 6.02). The N-terminal MGp42 sequence started from the 250th residue (Asp-250) of the deduced amino acid sequence and consisted of 386 amino acid residues; these results are consistent with those of 2D immunoblotting. MGp42 showed sequence similarity to members of the heat shock protein 70 (hsp70) family. Immunoblot inhibition tests revealed no IgE cross-reactivity between MGp42 and human HSP70.

CONCLUSIONS:

MGp42 may be a cleavage product of intact HSP70. This novel M. globosa allergen could be useful for the diagnosis of AD.

PMID:
19540092
DOI:
10.1016/j.jdermsci.2009.05.005
[Indexed for MEDLINE]

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