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Biotechniques. 1991 Jul;11(1):102-4, 106, 108-9.

Application of a novel chemiluminescence-based DNA detection method to single-vector and multiplex DNA sequencing.

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1
New England BioLabs.

Abstract

A chemiluminescent DNA detection method is described and its application shown for both single-vector and multiplex DNA sequencing using the standard dideoxy chain-termination process. This recently developed detection method, which utilizes the light emitted by an enzyme-catalyzed dioxetane reaction, is highly sensitive and affords significant advantages in safety and speed over the traditional radioactive labeling method. When adapted to a multiplex strategy, this chemiluminescent detection method constitutes a safe, simple and rapid method for increasing the throughput of DNA sequencing procedures.

PMID:
1954008
[Indexed for MEDLINE]
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