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Chem Biol Interact. 2009 Aug 14;180(3):506-14. doi: 10.1016/j.cbi.2009.03.014. Epub 2009 Mar 27.

Anti-inflammatory effect of 4-O-methylhonokiol, compound isolated from Magnolia officinalis through inhibition of NF-kappaB [corrected].

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College of Pharmacy, CBITRC, Chungbuk National University, 12, Gaesin-dong, Heungduk-gu, Cheongju, Chungbuk 361-763, Republic of Korea.

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  • Chem Biol Interact. 2010 Dec 5;188(3):677.


The bioactive constituents isolated from the bark of Magnolia officinalis such as magnolol, honokiol and obovatol have anti-inflammatory properties through the inactivation of NF-kappaB which is an important factor in the regulation of inflammatory reaction. We recently isolated neolignan compound, 4-O-methylhonokiol, from M. officinalis. In the present study, we investigated whether or not 4-O-methylhonokiol inhibits inflammatory reaction through the inhibition of NF-B activity [corrected]. The results showed that 4-O-methylhonokiol (2.5-10 microM) inhibited LPS (1 microg/ml)-induced NO generation in macrophage Raw 264.7 cells in a concentration-dependent manner with IC(50) value 9.8 microM. The inhibition of NO generation by 4-O-methylhonokiol was consistent with the inhibitory effect on the expression as well as transcriptional activity of inducible nitric oxide synthase (iNOS). In addition, 4-O-methylhonokiol inhibited the LPS-induced transcriptional and DNA binding activities of NF-kappaB as well as p50 and p65 translocation into the nucleus. Topical application of 4-O-methylhonokiol (0.1-1 mg/ear) inhibited 12-O-tetradecanoylphorbol-13-acetate-induced inflammatory ear edema formation, NF-kappaB activity, and iNOS and COX-2 expression. The present results provided evidence that 4-O-methylhonokiol has anti-inflammatory properties through inhibition of the NF-kappaB pathway, and suggested that 4-O-methylhonokiol can be used as an anti-inflammatory agent.

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