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Lab Chip. 2009 Jul 7;9(13):1836-9. doi: 10.1039/b901327j. Epub 2009 May 20.

Resonant Rayleigh light scattering response of individual Au nanoparticles to antigen-antibody interaction.

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Nano-optics and Biomolecular Engineering National Laboratory, Department of Chemical Engineering, Sungkyunkwan University, Suwon, 440-746, Korea.


A proof-of-concept study was reported on analysis of antigen-antibody recognition based on resonant Rayleigh scattering response of single Au nanoparticles in an imaging chamber. As benefited by a traditional dark-field microscope and a spectrograph, individual Au nanoparticles (30 nm) were observed with high signal-to-noise ratio and they were effectively utilized to monitor changes in refractive index induced by specific binding of the adsorbates. Using PSA antigen as a model, a LSPR lambda(max) shift of about 2.85 nm was recorded for a molecular binding corresponding to 0.1 pg ml(-1) of the protein biomarker. This result successfully demonstrates a non-labeling detection system for proteins as well as thousands of different chemical or biological species, and it possesses a great potential as a sensitive, on-chip and multiplexing detection.

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