Send to

Choose Destination
Opt Express. 2007 Mar 5;15(5):2459-67.

4Pi microscopy of type A with 1-photon excitation in biological fluorescence imaging.


We demonstrate that oil immersion lenses with a semi-aperture angle >/= 74 degrees enable 4Pi confocal fluorescence microscopy of type A with 1 photon excitation. The axial sidelobes amount to < 50 % of the main diffraction maximum, implying that lobe induced artifacts can be removed from the image data. The advancement reported herein enables a relative inexpensive implementation of 4Pi microscopy, providing axially superresolved 3D-imaging in transparent samples. As an example, we show dual-color 4Pi images of double stained Golgi stacks in a mammalian cell with 110 nm axial resolution. The resolution can be further enhanced to values slightly below 100 nm by image deconvolution.


Supplemental Content

Full text links

Icon for Optical Society of America
Loading ...
Support Center