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J Exp Clin Cancer Res. 2009 Jun 16;28:82. doi: 10.1186/1756-9966-28-82.

Down-regulated miR-9 and miR-433 in human gastric carcinoma.

Author information

1
Department of Gastroenterology, The Second Affiliated Hospital, Chongqing Medical University, Chongqing 400010, PR China. wdlz129@sina.com.

Abstract

BACKGROUND:

MircoRNAs(miRNAs) are short, endogenously non-coding RNAs. The abnormal expression of miRNAs may be valuable for the diagnosis and treatment of tumors.

METHODS:

To screening the special miRNAs in gastric carcinoma, expression level of miRNAs in gastric carcinoma and normal gaster samples were detected by miRNA gene chip. Then, the expressions of miR-9 and miR-433 in gastric carcinoma tissue and SGC7901 cell line were validated by qRT-PCR. GRB2 and RAB34, targets of miR-433 and miR-9 respectively, were detected by Western blot.

RESULTS:

We found 19 miRNAs and 7 miRNAs were down-regulated and up-regulated respectively. Compared with normal gaster samples, our data showed that miR-9 and miR-433 were down-regulated in gastric carcinoma. Meanwhile, we also found that miR-433 and miR-9 regulated the expression levels of GRB2 and RAB34 respectively.

CONCLUSION:

Our data show miR-9 and miR-433 was down-regulated in gastric carcinoma. The targets of miR-433 and miR-9 were tumor-associated proteins GRB2 and RAB34 respectively. This result provided the related information of miRNAs in gastric carcinoma.

PMID:
19531230
PMCID:
PMC2739520
DOI:
10.1186/1756-9966-28-82
[Indexed for MEDLINE]
Free PMC Article

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