Send to

Choose Destination
Opt Express. 2006 Dec 11;14(25):12217-29.

FRET-FLIM at nanometer spectral resolution from living cells.


We report the investigation of Foerster's Resonance Energy Transfer dynamics in GFP based tandem constructs in living T-cells using a combination of Fluorescence Lifetime Imaging Microscopy (FLIM) and Fluorescence Lifetime Micro-Spectroscopy (FLMS) at picosecond time resolution and nanometer spectral resolution. The involvement of multiple lifetimes of CFP in energy transfer was analyzed by plotting pre-exponential factors of individual lifetimes along the wavelength resulting in the Decay Associated Spectra (DAS). A change in the amplitude of pre-exponential factors from positive to negative at the acceptor emission maxima was used as a confirmation of FRET in the multiexponential lifetime analysis.


Supplemental Content

Full text links

Icon for Optical Society of America
Loading ...
Support Center