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Opt Express. 2006 Oct 30;14(22):10907-12.

Volumetric imaging of holographic optical traps.

Abstract

The holographic optical trapping technique creates arbitrary three-dimensional configurations of optical traps, each with individually specified characteristics. Holographic modification of the individual traps' wavefronts can transform conventional point-like optical tweezers into traps with different structures and properties, and can position them independently in three dimensions. Here, we describe a technique for rapidly characterizing holographic optical traps' three-dimensional intensity distributions. We create volumetric representations by by holographically translating the traps through the optical train's focal plane, acquiring a stack of two-dimensional images in the process. We apply this technique to holographic line traps, which are used to create tailored one-dimensional potential energy landscapes for mesoscopic objects. These measurements highlight problems that can arise when projecting extended traps with conventional optics and demonstrates the effectiveness of shape-phase holography for creating nearly ideal line traps.

PMID:
19529503
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