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J Dairy Sci. 2009 Jul;92(7):3014-8. doi: 10.3168/jds.2008-1702.

Effect of whey protein on the proliferation and differentiation of osteoblasts.

Author information

1
Key Lab of Dairy Science, Ministry of Education, Northeast Agricultural University, Harbin 150030, China. xr19801101@yahoo.com.cn

Abstract

Effects of whey protein on osteoblasts were evaluated. The whey protein was added to the culture medium at concentrations of 0.02 and 0.1 mg/mL. In vitro, whey protein stimulated the proliferation and differentiation of osteoblasts cultured in different concentrations of whey protein. The levels of osteocalcin and insulin-like growth factor-I in the culture medium also increased. Real-time reverse transcription-PCR results showed that the mRNA expression of osteoprotegerin (OPG) and receptor activator of nuclear factor-kappa B ligand (RANKL) increased in the cells in a dose-dependent manner, and when the results were expressed as OPG/RANKL ratio, a significant increase could be seen in the 0.1 mg/mL whey protein group. These results showed that the active component in the whey protein plays an important role in bone formation and a potential therapeutic role in osteoporosis by activating osteoblasts.

PMID:
19528578
DOI:
10.3168/jds.2008-1702
[Indexed for MEDLINE]

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