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Nat Methods. 2009 Jul;6(7):493-5. doi: 10.1038/nmeth.1342. Epub 2009 Jun 14.

Agouti C57BL/6N embryonic stem cells for mouse genetic resources.

Author information

1
Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK.

Abstract

We report the characterization of a highly germline competent C57BL/6N mouse embryonic stem cell line, JM8. To simplify breeding schemes, the dominant agouti coat color gene was restored in JM8 cells by targeted repair of the C57BL/6 nonagouti mutation. These cells provide a robust foundation for large-scale mouse knockout programs that aim to provide a public resource of targeted mutations in the C57BL/6 genetic background.

PMID:
19525957
PMCID:
PMC3555078
DOI:
10.1038/nmeth.1342
[Indexed for MEDLINE]
Free PMC Article

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