Format

Send to

Choose Destination
Nat Neurosci. 2009 Jul;12(7):947-53. doi: 10.1038/nn.2345. Epub 2009 Jun 14.

Twin-spot MARCM to reveal the developmental origin and identity of neurons.

Author information

1
Department of Neurobiology, University of Massachusetts, Worcester, USA.

Abstract

A comprehensive understanding of the brain requires the analysis of individual neurons. We used twin-spot mosaic analysis with repressible cell markers (twin-spot MARCM) to trace cell lineages at high resolution by independently labeling paired sister clones. We determined patterns of neurogenesis and the influences of lineage on neuron-type specification. Notably, neural progenitors were able to yield intermediate precursors that create one, two or more neurons. Furthermore, neurons acquired stereotyped projections according to their temporal position in various brain sublineages. Twin-spot MARCM also permitted birth dating of mutant clones, enabling us to detect a single temporal fate that required chinmo in a sublineage of six Drosophila central complex neurons. In sum, twin-spot MARCM can reveal the developmental origins of neurons and the mechanisms that underlie cell fate.

PMID:
19525942
PMCID:
PMC2701974
DOI:
10.1038/nn.2345
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Nature Publishing Group Icon for PubMed Central
Loading ...
Support Center