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Food Chem Toxicol. 2009 Oct;47(10):2401-6. doi: 10.1016/j.fct.2009.06.010. Epub 2009 Jun 9.

Anti-obesity effect of sulfated glucosamine by AMPK signal pathway in 3T3-L1 adipocytes.

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1
Marine Bioprocess Research Center, Pukyong National University, Busan 608-737, Republic of Korea.

Abstract

In this study, we investigated the effect of sulfated glucosamine (SGlc) on adipogenesis of 3T3-L1 adipocytes during differentiation of preadipocytes into adipocytes by measuring lipid accumulation and adipogenesis related factors. Treatment with SGlc reduced the triglyceride content in Oil-Red O staining and enhanced glycerol secretion in adipocytes in a dose-dependent manner. In addition, SGlc induced the down-regulation of adipogenesis related factors and adipocyte specific gene promoters. Moreover, treatment of 3T3-L1 adipocytes with SGlc activated the phosphorylated adenosine monophosphate-activated protein kinase (AMPK) alpha and beta along with their substrate, acetyl-CoA carboxylase (ACC). These results suggest that inhibitory effect of SGlc on adipocyte differentiation might be mediated through the up-regulation of AMPK pathway.

PMID:
19520133
DOI:
10.1016/j.fct.2009.06.010
[Indexed for MEDLINE]
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