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Opt Express. 2006 Apr 17;14(8):3467-71.

Two-photon bioimaging with picosecond optical pulses from a semiconductor laser.

Abstract

Toward a practical light source for two-photon bioimaging, we have generated kilowatt peak power of 0.77 mum wavelength and 5 ps optical pulse via second-harmonic generation of the amplified output from a gain-switched 1.55 mum semiconductor laser. This compact scheme and stable optical-pulse-source has been successfully used for the two-photon fluorescence bioimaging of actin filaments in PtK2 cells.

PMID:
19516492

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