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Methods Mol Biol. 2009;552:199-211. doi: 10.1007/978-1-60327-317-6_14.

BacMam: versatile gene delivery technology for GPCR assays.

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1
Molecular Discovery Research, Biological Reagents and Assay Development, GlaxoSmithKline R&D, Collegeville, PA, USA.

Abstract

BacMam viruses are modified baculoviruses that contain mammalian expression cassettes for viral gene delivery and transient expression in mammalian cells. They are easily, inexpensively, and rapidly generated and provide a versatile solution for G protein-coupled receptor (GPCR) cell-based assay development. Using BacMam technology, target gene expression levels are easily controlled and simultaneous delivery of multiple genes is possible, for example, coexpression of a receptor and a G protein or a reporter gene. BacMam viruses are compatible with the GPCR cell-based assay formats typically used in high-throughput screening and provide an unparalleled level of experimental flexibility that is simply not possible when using stable recombinant cell lines.

PMID:
19513651
DOI:
10.1007/978-1-60327-317-6_14
[Indexed for MEDLINE]
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