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J Biophotonics. 2009 Jul;2(6-7):380-8. doi: 10.1002/jbio.200910021.

Dual optical coherence tomography/fluorescence microscopy for monitoring of Drosophila melanogaster larval heart.

Author information

1
Applied Optics Group, School of Physical Sciences, University of Kent, CT2 7NH Canterbury, UK. a.bradu@kent.ac.uk

Abstract

This article demonstrates a combined instrument of two imaging modalities to acquire information on cardiac function in larval Drosophila melanogaster: optical coherence tomography (OCT) and laser scanning fluorescence microscopy (LSFM). For this purpose, a dedicated imaging instrument able to sequentially provide cross-sectional OCT and C-scan LSFM images has been developed. With this dual-imaging system, the heart can be easily located and visualized within the specimen and the change of the heart shape in a cardiac cycle can be monitored.

PMID:
19504517
DOI:
10.1002/jbio.200910021
[Indexed for MEDLINE]
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