Relationships between single nucleotide polymorphisms of the H-FABP gene and slaughter and meat quality traits in chicken

Biochem Genet. 2009 Aug;47(7-8):511-20. doi: 10.1007/s10528-009-9249-z. Epub 2009 Jun 7.

Abstract

Using PCR-SSCP with five primer pairs, we detected six single nucleotide polymorphisms of the H-FABP gene: 332G --> A, 534G --> A, 783C --> T, 835C --> T, 1198T --> C, and 2329C --> T. Chi-square results showed significant differences (P < 0.05) in genotype frequency among breeds in Fragment 1 and extremely significant differences (P < 0.01) in Fragments 2-4. We found a significant association between Fragment 2 genotype and muscle fiber number, Arg and Thr (P < 0.05); between Fragment 3 genotype and living weight, carcass weight, breast muscle weight, abdominal fat weight, and abdominal fat percentage (P < 0.05); between Fragment 4 genotype and Thr, Phe, and inosinic acid (P < 0.05). It was concluded that H-FABP was the major gene influencing slaughter performance and meat quality or was linked with the major gene in these strains and that the C783T mutation could be used as a candidate molecular genetic marker for breeding selection. The combination M1C2-B2B2-D1D1 is an ideal model for breeding in these strains because it can improve slaughter and meat quality traits.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Abattoirs
  • Animals
  • Base Sequence
  • Breeding
  • Chickens / anatomy & histology*
  • Chickens / classification
  • Chickens / genetics*
  • Fatty Acid-Binding Proteins / genetics*
  • Female
  • Food Technology*
  • Gene Frequency
  • Genotype
  • Male
  • Meat*
  • Polymorphism, Single Nucleotide*

Substances

  • Fatty Acid-Binding Proteins