Format

Send to

Choose Destination
See comment in PubMed Commons below
Mol Cell Biochem. 2009 Dec;332(1-2):33-41. doi: 10.1007/s11010-009-0171-9. Epub 2009 Jun 6.

Akt is a direct target for myricetin to inhibit cell transformation.

Author information

  • 1United Graduate School of Agricultural Sciences, Kagoshima University, Korimoto 1-21-24, Kagoshima City, 890-0065, Japan.

Abstract

Akt, a serine/threonine kinase, is a critical regulator in many cellular processes including cell growth, proliferation, and apoptosis. In this study, we found that myricetin, a typical flavonol existing in many fruits and vegetables, could directly target Akt to inhibit cell transformation. Binding assay revealed that myricetin bound to Akt directly by competing with ATP. In vitro and ex vivo data confirmed that myricetin inhibited the phosphorylation and kinase activity of Akt. Molecular modeling suggested that myricetin easily docks to the ATP-binding site of Akt with hydrogen bonds. Signaling analysis data further demonstrated that myricetin inhibited Akt-mediated activator protein-1 (AP-1) transactivation, cyclin D1 expression and cell transformation. Overall, our results indicate that Akt is a direct target for myricetin to inhibit cell transformation.

PMID:
19504174
DOI:
10.1007/s11010-009-0171-9
[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Springer
    Loading ...
    Support Center