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Dev Biol. 2009 Aug 15;332(2):309-24. doi: 10.1016/j.ydbio.2009.05.575. Epub 2009 Jun 3.

Four distinct phases of basket/stellate cell migration after entering their final destination (the molecular layer) in the developing cerebellum.

Author information

1
Department of Neurosciences/NC30, Lerner Research Institute, The Cleveland Clinic Foundation, Cleveland, OH 44195, USA.

Abstract

In the adult cerebellum, basket/stellate cells are scattered throughout the ML, but little is known about the process underlying the cell dispersion. To determine the allocation of stellate/basket cells within the ML, we examined their migration in the early postnatal mouse cerebellum. We found that after entering the ML, basket/stellate cells sequentially exhibit four distinct phases of migration. First, the cells migrated radially from the bottom to the top while exhibiting saltatory movement with a single leading process (Phase I). Second, the cells turned at the top and migrated tangentially in a rostro-caudal direction, with an occasional reversal of the direction of migration (Phase II). Third, the cells turned and migrated radially within the ML at a significantly reduced speed while repeatedly extending and withdrawing the leading processes (Phase III). Fourth, the cells turned at the middle and migrated tangentially at their slowest speed, while extending several dendrite-like processes after having completely withdrawn the leading process (Phase IV). Finally, the cells stopped and completed their migration. These results suggest that the dispersion of basket/stellate cells in the ML is controlled by the orchestrated activity of external guidance cues, cell-cell contact and intrinsic programs in a position- and time-dependent manner.

PMID:
19500566
PMCID:
PMC2718556
DOI:
10.1016/j.ydbio.2009.05.575
[Indexed for MEDLINE]
Free PMC Article

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