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Science. 2009 Jun 5;324(5932):1323-7. doi: 10.1126/science.1170088.

McsB is a protein arginine kinase that phosphorylates and inhibits the heat-shock regulator CtsR.

Author information

1
Research Institute of Molecular Pathology, Dr. Bohrgasse 7, A-1030 Vienna, Austria.

Abstract

All living organisms face a variety of environmental stresses that cause the misfolding and aggregation of proteins. To eliminate damaged proteins, cells developed highly efficient stress response and protein quality control systems. We performed a biochemical and structural analysis of the bacterial CtsR/McsB stress response. The crystal structure of the CtsR repressor, in complex with DNA, pinpointed key residues important for high-affinity binding to the promoter regions of heat-shock genes. Moreover, biochemical characterization of McsB revealed that McsB specifically phosphorylates arginine residues in the DNA binding domain of CtsR, thereby impairing its function as a repressor of stress response genes. Identification of the CtsR/McsB arginine phospho-switch expands the repertoire of possible protein modifications involved in prokaryotic and eukaryotic transcriptional regulation.

PMID:
19498169
DOI:
10.1126/science.1170088
[Indexed for MEDLINE]
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