Format

Send to

Choose Destination
See comment in PubMed Commons below
Med Princ Pract. 2009;18(4):280-3. doi: 10.1159/000215724. Epub 2009 Jun 2.

Whole-blood polymerase chain reaction and restriction fragment length polymorphism: a simplified method by microwave irradiation.

Author information

1
Department of Medical Laboratory Sciences, Faculty of Allied Health Sciences, Kuwait University, Sulaibekhat 90805, Kuwait. mehrez@hsc.edu.kw

Abstract

OBJECTIVES:

The aim of the present study was to develop a simple, quick and cheap method to process whole-blood samples for the molecular techniques polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) without the use of expensive reagents or sophisticated machines.

MATERIALS AND METHODS:

Venous whole-blood samples were collected from 40 individuals. The samples were frozen at -80 degrees C, and then rapidly thawed at 37 degrees C. Each sample was incubated with distilled water, then boiled in a microwave and centrifuged. The supernatant was taken directly for PCR and RFLP. For comparison, PCR and RFLP were performed on DNA purified from the same samples using the phenol-chloroform method and two commercial DNA extraction kits.

RESULTS:

PCR/RFLP results using the presented method were qualitatively similar to those obtained by DNA extracted using the other three methods.

CONCLUSION:

The presented method proved to be a simpler and cheaper way of processing whole-blood samples for PCR and RFLP analyses.

PMID:
19494534
DOI:
10.1159/000215724
[Indexed for MEDLINE]
Free full text
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for S. Karger AG, Basel, Switzerland
    Loading ...
    Support Center