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J Virol. 2009 Aug;83(16):8270-5. doi: 10.1128/JVI.00670-09. Epub 2009 Jun 3.

Truncation of TRIM5 in the Feliformia explains the absence of retroviral restriction in cells of the domestic cat.

Author information

1
Institute of Comparative Medicine, University of Glasgow, United Kingdom. w.mcewan.1@research.gla.ac.uk

Abstract

TRIM5alpha mediates a potent retroviral restriction phenotype in diverse mammalian species. Here, we identify a TRIM5 transcript in cat cells with a truncated B30.2 capsid binding domain and ablated restrictive function which, remarkably, is conserved across the Feliformia. Cat TRIM5 displayed no restriction activity, but ectopic expression conferred a dominant negative effect against human TRIM5alpha. Our findings explain the absence of retroviral restriction in cat cells and suggest that disruption of the TRIM5 locus has arisen independently at least twice in the Carnivora, with implications concerning the evolution of the host and pathogen in this taxon.

PMID:
19494015
PMCID:
PMC2715776
DOI:
10.1128/JVI.00670-09
[Indexed for MEDLINE]
Free PMC Article

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