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Methods Mol Biol. 2009;544:253-67. doi: 10.1007/978-1-59745-483-4_16.

Raman-assisted X-ray crystallography for the analysis of biomolecules.

Author information

1
Institut de Biologie Structurale Jean-Pierre Ebel, 41 Rue Jules Horowitz, 38027, Grenoble Cedex, France. dominique.bourgeois@ibs.fr

Abstract

In this chapter, we describe Raman microspectrophotometry applied to crystals of biomolecules. Raman spectra collected in crystallo provide structural information highly complementary to X-ray diffraction, relate the crystalline state to the solution state, and allow the identification of ligand-bound or intermediate states of macromolecules. Nonresonant Raman spectroscopy is particularly suitable to the study of macromolecular crystals, and therefore applies to a wide range of noncolored crystalline proteins. Practical issues related to the investigation of crystals by Raman microspectrophotometry are reviewed, and the current limitations are highlighted.

PMID:
19488704
DOI:
10.1007/978-1-59745-483-4_16
[Indexed for MEDLINE]

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